||Order or Inquire for H1N1 ( Swine Flu 2009 ) HA ELISA Pair Set|
||First H1N1 ( Swine Flu 2009 ) HA ELISA Pair Set in the world|
||No cross-reactivity with H1N1 ( Seasonal Flu ) HA|
||Detection limit - 12.2 pg/ml|
||Affordable price and 30%-80% cost saving for Bulk order|
Capture Antibody - 0.2 mg/mL of mouse anti-2009 H1N1 hemagglutinin monoclonal antibody. Dilute to a working concentration of 2.0 μg/mL in CBS before coating.
Detection Antibody - 0.5 mg/mL of rabbit anti-2009 H1N1 hemagglutinin polyclonal antibody conjugated to horseradish-peroxidase ( HRP ). Dilute to a working concentration of 2 μg/mL in detection antibody dilution buffer before use.
Standard - Each vial contains 40 ng of recombinant H1N1 ( A/California/04/2009 ) hemagglutinin. Reconstitute with 1 mL detection antibody dilution buffer. A nine-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 780 pg/mL is recommended.
The minimum detectable dose of H1N1 hemagglutinin ( HA ) was determined to be approximately 12.2 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
The following Hemagglutinin of different influenza virus types and subtypes prepared at 200 ng/mL were tested and No cross-reactivity was identified:
Influenza B (B/Florida/4/2006)
The 2009 H1N1 influenza (swine flu) hemagglutinin ELISA Pair Set is for the quantitative determination of hemagglutinin.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for hemagglutinin coated on a 96-well plate. Standards and samples are added to the wells, and any hemagglutinin present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti- hemagglutinin polyclonal antibody is then added, producing an antibody-antigen-antibody “sandwich”. The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of hemagglutinin present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
STORAGE - Detection Antibody should be protected from prolonged exposure to light. Aliquot the reagents and store at -20℃ to -70℃ in a manual defrost freezer.
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
|A/Puerto Rico/8/34/Mount Sinai|
|A/Japanese white-eye/Hong Kong/1038/2006|
|A/Common magpie/Hong Kong/2256/2006|
|H5N2||A/American green-winged teal/California/HKWF609/07|
|A/Guinea fowl/Hong Kong/WF10/99|
Influenza (flu) is a viral respiratory infection in mammals and birds. This virus is divided into three main types (A, B and C). Influenza A is found in a wide variety of mammalian and avian species and is associated with the major human pandemics. Influenza B is largely confined to humans and became unexpectedly prevalent in humans during 2000-2002. Influenza C infects humans, dogs and pigs and generally causes only mild upper respiratory tract infection. However, influenza A and B viruses cause a wide spectrum of severe disease including lower respiratory, tract infection, pneumonia and encephalitis. Influenza A is further divided into subtypes based on antigenic differences in the membrane proteins hemagglutinin (HA) and neuraminidase (NA). 16 HAs (H1-H16) and 9 NA (N1-N9) had been identified. While different combinations of the two antigens appear more frequently in some groups of birds than others, only few subtypes have established themselves in humans (HA:H1, H2, and H3; NA: N1 and N2).
The 2009 flu pandemic is caused by a new swine-origin influenza A (H1N1) virus which is a recombinated production by human H3N2, swine H1N1 and avian H5N1strains. The mixing of new genetic elements in swine can result in the emergence of viruses with pandemic potential in humans. As 2009 H1N1 influenza is a new virus and most people have no or little immunity this virus could cause more infections than are seen with seasonal flu. The virus spread worldwide by human-to-human transmission, causing the World Health Organization to raise its pandemic alert to the highest level 6. The HA, NA, and MP sequences of 2009 H1N1 flu (swine flu) have been placed on deposit at GISAID.
Hemagglutinin (HA), which binds to sialic acid (SA)-containing receptors on host cells, is the protein that produces neutralizing antibodies. Hemagglutinin plays a major role in the determination of host range restriction and virulence because human influenza HA preferentially binds to SA-α-2,6 while avian influenza HA preferentially binds to SA-α-2,3. The cleavage of HA into two disulfide-linked subunits, HA1 and HA2, is a prerequisite for initiating infection. Usually HA is restricted to be cleaved at respiratory tracts by limited proteases. Highly pathogenic avian influenza contains a stretch of basic residues adjacent to the HA cleavage site, enabling its HA to be cleaved by a wide range of proteases with ubiquitous tissue distributions. This process permits productive virus replication in organs outside of the respiratory and gastrointestinal tracts, including the brain, resulting in widespread disease and high mortality rates.
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