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BroadDyeTM Terminator V3.5 Cycle Sequencing Kit
The BroadDyeTM Terminator V3.5 Cycle Sequencing Kit provides pre-mixed reagents for Sanger sequencing reactions.
Description:
This kit uses four different fluorescent dyes to label ddNTPs, which are added sequentially to the primer through a cycle sequencing reaction. The kit reagents are suitable for performing fluorescence-based cycle sequencing reactions on single-stranded or double-stranded DNA templates, on PCR fragments, and on large templates (for example, BAC clones).
BroadDyeTM terminator kit is compatible with commercially available BigDye terminator V3.1 kit, without any changes in the workflow.
Features:
With enhanced robustness and better dye mobility characteristics, the BroadDyeTM Terminator V3.5 Cycle Sequencing Kit can be used in a wide range of applications with the following features:
· Long read lengths.
· Better performance reading through GC rich regions.
· Longer reads with more uniform peak heights.
· Improved productivity for less cost.
Applications:
· De novo sequencing
· Resequencing
· Sequencing difficult templates
· Long-read sequencing
· Sequencing across all template types (plasmids, PCR products)
· Mixed-base detection
· Sequencing short PCR products using rapid electrophoresis run modules
BroadDyeTM Terminator V3.5 Cycle Sequencing Kit contents-Standard
|
Contents |
Quantity × Specification |
Cat. No. |
Storage |
|
BroadDyeTM Terminator v3.5 Ready Reaction Mix |
2×20ml |
35001 |
Store at -15 to -25℃ |
|
BroadDyeTM Terminator v3.5 5X Sequencing buffer |
4×30ml |
35002 |
Store at 4℃ |
• 3500/3500xL Genetic Analyzer
• 3130/3130xl Genetic Analyzer
• 310 Genetic Analyzer
Template Compatibility:
Plasmid DNA (≤15Kb)
Single Stranded DNA
Lambda DNA
Genomic DNA (Bacterial)
PCR Amplicons
Storage: Store kit at -20°C. Avoid repeated freeze-thaw cycles.
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.

| Registration Date | 2009/07/23(Year/Month/Date) |
|---|---|
| Buyer / Seller in EC21 | Seller |
| Business Type | Manufacturer |
| Year established | 2020 |
| Employees total | 51 - 100 |
| Annual revenue | USD 1,000,001 - 2,000,000 |
| Company | Shanghai Rnaera Technology Co., Ltd |
|---|---|
| Address | 1103, Building 16, Lane 758, Ronghui Road, Songjiang District, Shanghai, ChinaShanghaiShanghai201613China |
| Phone | 86 - 021 - 11111111 |
| Fax | 86 - 021 - 11111111 |
| Homepage | https://www.made-in-china.com/showroom/vivianzheng01 |
| Contact | vivian zheng / Sales Staff |