Human Adenosine Triphosphate ELISA Kit

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Supplier Info.

Company Name Bluegene Biotech
Membership Free member
Registration Date2023.05.05
Country/RegionChina
CityShanghai
Contact Vicky Cheng(Mr.)

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Product Attributes

- Place of Origin China
- Supply Type OEM Service
- Condition New
- Related Keywords Human Adenosine Triphosph

Description by Manufacturer

E01A0038 Human Adenosine Triphosphate ELISA kit

Human Adenosine Triphosphate ATP ELISA kit is suitable for the detection of samples from human species. Adenosine Triphosphate can also be called Adenosine-5'-Triphosphate, ATP.

Specifications of Human Adenosine Triphosphate ATP ELISA kit

Product Information
Cat. No.	E01A0038
Product Name	Human Adenosine Triphosphate ELISA kit
Species	Human
Product Size	48 Tests / 96 Tests
Concentration	50-1000 ng/mL
Sensitivity	1.0 ng/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Principle of the Assay

ATP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ATP antibody and an ATP-HRP conjugate. The assay sample and buffer are incubated together with ATP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATP concentration since ATP from samples and ATP-HRP conjugate compete for the anti-ATP antibody binding site. Since the number of sites is limited, as more sites are occupied by ATP from the sample, fewer sites are left to bind ATP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATP concentration in each sample is interpolated from this standard curve.

Quality Control on Human Adenosine Triphosphate ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	94-106%
Linearity	Diluent Ratio	Range %
	1:02	93-101
	1:04	90-107
	1:08	92-105
Specificity/Cross-reactivity	No significant cross-reactivity or interference between ATP and analogues was observed.

Citations of Human Adenosine Triphosphate ELISA kit

E01A0038 has been referenced in the below publications:

The research of the metabolism and in vivo functional changes of erythrocyte stored at -65℃.

Elisa kit supplier Shanghai BlueGene Biotech is a company with research, production, and sale in one, our product's range includes Enzyme-Linked Immunosorbent Assay (ELISA) kits and registered trademark "cgbio®" ELISA Kits, proteins, antibodies, plasmids, and other reagents, such as His-tag Protein Purification kit.

If you want to know more kinds of human insulin elisa kit , please visit our website.

Price

Price US$ 300 / Piece

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Company Profile

About Bluegene Biotech

Membership Free member
Registration Date2023.05.05
Country/RegionChina
CityShanghai
Contact Vicky Cheng(Mr.)

BlueGene Biotech is a professional ELISA kits manufacturer with 12 years of experience, which focuses on life sciences products and biotechnology. We're equipped with an advanced ELISA test research lab, offering more than 100,000 ELISA kits, about 2000 published academic papers cited the usage of Bluegene Biotech ELISA kits. Our products cover research areas including adipokines, cancer, cardiovascular, cell biology, development, endocrinology, immunology, microbiology, neurobiology, stem cell, and ubiquitin. Our distributors are from all over the world, we're able to provide our products and services to many countries in America, Australia, Africa, Asia, and Europe.

Selling Categories

- Chemicals >> Other Organic Chemicals

Keywords

cho hcp elisa kit

Registration Date	2023/05/05(Year/Month/Date)
Buyer / Seller in EC21	Seller
Business Type	Manufacturer
Year established	2007
Employees total	51 - 100
Annual revenue	Less than USD 100,000

Contact Information Order Credit Report

Company	Bluegene Biotech
Address	Lane 166 Tianxiong Road(Rm.403,404, Bldg 2),Pudong NewDistrict,ShanghaiShanghaiShanghai201318China
Phone	86 - 021 - 8826373
Homepage	www.elisakit.cc/
Contact	Vicky Cheng / Marketing Manager

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Human Adenosine Triphosphate ELISA Kit

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