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Neomycin ELISA test Kit
Catalog No. LSY-10005
1. Principle
This test kit is based on the competitive enzyme immunoassay. The coupling antigen is pre-coated on the micro-well stripes. The Neomycin residues in the sample and the coupling antigen pre-coated on the micro-well stripes compete for the anti-Neomycin antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Neomycin in it. This value is compared to the standard curve and the Neomycin concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.5ppb
Incubation Temperature: 25℃
Incubation Time: 60min—15min
Detection limit
Tissue(Chicken/liver, pork/liver, shrimp, fish) 10ppb
Honey 10ppb
Milk, milk powder 10ppb
Recovery rate
Tissue (chicken, pork, fish, shrimp), honey 85±19%
Liver (chicken or porcine) 70±17%
Milk, milk powder 85±16%
Cross-reaction rate
Neomycin 100%
Streptomycin < 0.1%
Gentamicin < 0.1%
Dihydrostreptomycin < 0.1%
Kanamycin < 0.1%
Tobramycin < 0.1%
Sisomicin < 0.1%
| 1 | Micro-well strips | 12 strips with 8 removable wells each |
|
| 2 | 6× standard solution (1 mL each) | 0ppb | 0.5ppb |
| 1.5ppb | 4.5ppb | ||
| 13.5ppb | 40.5ppb | ||
| 3 | Enzyme conjugate | 7ml | red cap |
| 4 | Antibody working solution | 10ml | blue cap |
| 5 | Substrate A | 7ml | white cap |
| 6 | Substrate B | 7ml | black cap |
| 7 | Stop solution | 7ml | yellow cap |
| 8 | 20× concentrated washing buffer | 40ml | white cap |
| 9 | 2× concentrated redissolving solution | 50ml | transparent cap |
| Registration Date | 2011/08/16(Year/Month/Date) |
|---|---|
| Buyer / Seller in EC21 | Seller |
| Business Type | Manufacturer |
| Year established | 2003 |
| Employees total | 51 - 100 |
| Annual revenue | USD 1,000,001 - 2,000,000 |
| Company | Shenzhen Lvshiyuan Biotechnology Co.,Ltd |
|---|---|
| Address | D building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, LonggangShenzhenGuangdong518120China |
| Phone | 86 - 755 - 28438788 |
| Fax | 86 - 755 - 28938800 |
| Contact | Bella Zou / Overseas sales |