Transscript All-in-one First-strand Cdna Synthesis Supermix for Qpcr(One-step Gdna Removal)

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Supplier Info.

Company Name Beijing TransGen Biotech Co., Ltd.
Membership Free member
Registration Date2014.02.12
Country/RegionChina
CityBeijing
Contact Dan Pan(Ms.)

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Product Attributes

- Brand Name TransGen-Transx-script
- Model Number AT341
- Place of Origin China
- Purity 99.9%
- Related Keywords cDNA Synthesis, qPCR Reagent, Molecular Biology Reagent

Description by Manufacturer

Ordering Information:

AT341-01	50 rxns (20 μl per reaction)

AT341-01

50 rxns (20 μl per reaction)

Storage: at -20°C for one year

Description

This supermix provides all the necessary components for cDNA synthesis from total RNA or mRNA. It is provided at 5× concentration and used at 1× concentration by adding gDNA remover, RNA and H₂O. Simultaneous genomic DNA removal and cDNA synthesis are performed in one tube. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85^oC for 5 seconds. . The resulting cDNA are suitable for qPCR and not for regular PCR.

Advantages

• Simultaneous genomic DNA removal and cDNA synthesis in one tube

• The optimal ratio of oligo(dT)18 primer to random primer(N9) for qPCR ready cDNA.

• qPCR ready cDNA in 15 minutes.

• cDNA up to 250 bp.

Application

Multiple copy and low copy gene synthesis

Kit Contents

Components	AT341-01 (50 rxns )
5×TransScript^®All-in-One SuperMix for qPCR	200 μl
5×TransScript^® All-in-One No-RT Control SuperMix for qPCR	20 μl
gDNA Remover	50 μl
RNase-free Water	1 ml

Procedures

Genomic DNA removal and first-strand cDNA synthesis

1. Reaction Components

Component	Volume
Total RNA/mRNA	*
5×TransScript^® All-in-One SuperMix for qPCR	4 μl
gDNA Remover	1 μl
RNase-free Water	to 20 μl

*Total RNA≤1 μg, mRNA≤100 ng (for 20 μl reaction system)

Optional: for higher efficiency, suggest to mix RNA and water first. Incubate the mix at 65^oC for 5 minutes, ice for 2 minutes. Then add other components.

2. Incubate at 42^oC for 15 minutes.

3. Incubate at 85^oC for 5 seconds to inactive enzymes.

RT-PCR

Reaction Components

Component	Volume	Final Concentration
cDNA	Variable	as required
Forward Primer (10 μM)	0.4 μl	0.2 μM
Reverse Primer (10 μM)	0.4 μl	0.2 μM
2×TransStart^® Green qPCR SuperMix	10 μl	1×
Passive Reference Dye	0.4 μl	1×
ddH₂O	Variable	-
Total Volume	20 μl	-

Price

Price Condition FOB

Packaging & Shipping

- Minimum Order1

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Transscript All-in-one First-strand Cdna Synthesis Supermix for Qpcr(One-step Gdna Removal)

CDNA Synthesis Related Products

Company Profile

About Beijing TransGen Biotech Co., Ltd.

Membership Free member
Registration Date2014.02.12
Country/RegionChina
CityBeijing
Contact Dan Pan(Ms.)

Beijing TransGen Biotech Co., Ltd. is a researcher, developer, manufacturer and marketer of more than 400 molecular, protein and cell biology products and kits for the life science research and diagnostics.

In 2001, our company was founded by three scientists, who have more than 40 years' combined life science research experience and more than 50 publications, with a mission to produce innovative and cost-effective products for the life science research. On March 21, 2006, TransGen Biotech was incorporated in Beijing, China. Our company's headquarters and manufacturing facilities are located in Beijing.

To date, our company has employed more than 100 employees in Beijing, and has more than 30 distribution centers covering almost all major cities in China. Our rich R&D experience and state-of-the-art facilities enable us to keep generating the most innovated and the highest quality products. From 2006 and 2009, our company was consecutively awarded as "High Tech Corporation in Beijing" by Beijing Local Government.

Currently, our products cover: Plasmid based DNA markers, high efficiency chemically competent cells, 5-minute PCR product cloning, a variety of PCR enzymes, RNase H deficient and high-temperature RT enzymes, one-step and two-steps qPCR enzymes, the highest efficiency mutagenesis kits, high quality nucleic acid extraction and purification kits, 5-minute expression vectors, unstained and prestained protein markers, Western Blot markers, and transfection reagents.

As a leading bioreagent company in China, we are looking forward to partnerships with you in your quest for ground-breaking life science discoveries.

Selling Categories

- Chemicals >> Chemical Reagents
- Chemicals >> Resin
- Chemicals >> Other Chemicals

Keywords

molecular biology reagent , cell biology reagent

Registration Date	2014/02/12(Year/Month/Date)
Buyer / Seller in EC21	Seller
Business Type	Manufacturer
Year established	2006
Employees total	101 - 500
Annual revenue	More than 100,000,000

Contact Information Order Credit Report

Company	Beijing TransGen Biotech Co., Ltd.
Address	F 4-5, Building B-3, No.66 Xixiaokou Road, Dongsheng Technology Park, Haidian DistrictBeijingBeijing100192China
Phone	0086 - 10 - 57815087
Fax	86 - 010 - 57815000
Homepage	http://www.transgenbiotech.com
Contact	Dan Pan

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Transscript All-in-one First-strand Cdna Synthesis Supermix for Qpcr(One-step Gdna Removal)

Product Attributes

Description by Manufacturer

Price

Packaging & Shipping

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