Beta-Lactam ELISA Test Kit

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Price

US$ 200

Min Order Quantity2 Box
Delivery Lead Time7
Payment Terms 1
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Supplier Info.

Company Name Reagen
Membership Free member
Registration Date2013.05.21
Country/RegionUnited States
CityMoorestown
Contact Sia

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Product Attributes

- Brand Name reagen
- Model Number RND99027
- Place of Origin United States
- Supply Type In-Stock Items
- Condition New
- Purity 95%
- Related Keywords Beta-Lactam, ELISA test Kit

Description by Manufacturer

Product Description

REAGEN^™ Beta-Lactam ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of beta-lactams including ampicillin, penicillin G, amoxicillin, cloxacillin, oxacillin in milk, urine, tissue (liver, kidney, and muscle), serum, and plasma samples. REAGEN^™ Beta-lactam ELISA Test Kit enables international and government regulatory agencies, food manufacturers and processors, as well as quality assurance organizations, to detect beta-lactam containing antibiotics to satisfy customer concerns about food safety.

The unique features of the kit are:

High recovery (75-105%), low-cost rapid sample prep protocol (10 minutes - 1 hour)
High sensitivity: 0.4 ng/gram = 0.4 ppb (parts per billion).
High reproducibility (interplate and intraplate CV typically <8%)
Rapid assay protocol (less than 2 hours to process 40 samples in duplicate)

Procedure Overview

The method is based on a competitive colorimetric ELISA assay. The drug of interest (beta-lactam antibiotic) has been coated in the plate wells. The sample to be tested is mixed with a high-affinity beta-lactam capture protein and added to the well. If the beta-lactam target is present in the sample, it will compete for the capture protein, thereby preventing the capture protein from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the capture protein bound to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample. The levels of beta-lactam antibiotic in the sample are determined by comparison of the color intensity of unknown samples with the standard curve, which is made using the beta-lactam standard provided in the kit.

Kit Contents, Storage and Shelf Life

Kit Contents	Amount	Storage
Beta-Lactam Coated Plate	1 x 96-well Plate (8 wells x 12 strips)	2-8°C
Penicillin G powder stock (1000 ng): Empty Vials for Standards: Negative control (white cap tube) 0.04 ng/mL (yellow cap tube) 0.08 ng/mL (orange cap tube) 0.2 ng/mL (pink cap tube) 0.3 ng/mL(purple cap tube) 0.6 ng/mL (blue cap tube)	2 1 1 1 1 1 1	2-8°C *
10X Beta-Lactam Capture Protein	1.8 mL	2-8°C *
TMB Substrate **	10 mL
200X HRP-Conjugated Antibody #2	100 mL
Antibody #2 Diluent **	20 mL
Stop Buffer **	20 mL
20X Wash Solution **	30 mL
10X Beta-Lactam Extraction Buffer* Milk Standard Dilution Buffer 1	2 x 28 mL 15 mL

* If the kit is to be stored for over two weeks before use, store Standard Dilution Buffer1, Penicillin G powder stock, 10X Beta-Lactam Capture Protein and 100X HRP-Conjugated Antibody #2 at -20°C in a freezer that does not cycle to higher temperatures (i.e. a non-frost-free freezer). To avoid repeated freeze-thawing of the capture protein it is strongly recommended to store this component in single-use aliquots.

Sensitivity

Antibiotic	Milk, Meat, Tissue, Fish and Urine(ppb)	Serum and Plasma(ppb)
Penicillin G	0.40	0.80
Ampicillin	0.43	0.86
Azlocillin	0.40*	0.80
Pipercillin	0.52	1.04
Nafcillin	0.58	1.16
Amoxicillin	0.62	1.24
Cloxacillin	0.64	1.28
Oxacillin	0.64	1.28
Cefoperazone	0.67	1.34
Ceftiofur	0.7	1.4

Price

Price US$ 200 / Box

Packaging & Shipping

- Packingcolor box
- Delivery Lead Time7
- Minimum Order 2 Box
- Total quantity in Stock11 – 20
- Shipping Career

Product Image

Beta-Lactam Related Products

Company Profile

About Reagen

Membership Free member
Registration Date2013.05.21
Country/RegionUnited States
CityMoorestown
Contact Sia

REAGEN LLC is an indrustry-leading biomedical enterprise focused on designing and producing a broad range of ELISA kits, including clinical diagnostic kits, food and feed safety kits, and veterinary disease kits. we also manufacture microplate readers, plate washers, and related instruments and equipment.

Selling Categories

- Chemicals >> Chemical Reagents

Keywords

elisa kit , elisa test kit

Registration Date	2013/05/21(Year/Month/Date)
Buyer / Seller in EC21	Seller
Business Type	Manufacturer
Year established	2008
Employees total	101 - 500
Annual revenue	USD 100,000 - 500,000

Contact Information Order Credit Report

Company	Reagen
Address	102 Commerce Dr. Unit 8, Moorestown, http://www.reagen.usMoorestownColorado08057United States
Phone	1 - 857 - 7270251
Fax	1 - 857 - 7270251
Homepage	http://WWW.reagen.us
Contact	Sia / mananger

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Beta-Lactam ELISA Test Kit

Product Attributes

Description by Manufacturer

Product Description

Procedure Overview

Kit Contents, Storage and Shelf Life

Sensitivity

Price

Packaging & Shipping

Product Image

Beta-Lactam Related Products

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Company Profile

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