Sulfonamide ELISA Test Kit

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PortMOORESTOWN
Delivery Lead Time7 days
Payment Terms 1

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Supplier Info.

Company Name Reagen
Membership Free member
Registration Date2013.05.21
Country/RegionUnited States
CityMoorestown
Contact Sia

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Product Attributes

- Brand Name reagen
- Model Number RND99035
- Place of Origin United States
- Supply Type In-Stock Items
- Condition Used - good
- Purity 95%
- Related Keywords Sulfonamide, ELISA Test Kit

Description by Manufacturer

Product Description

REAGEN^™Sulfonamide ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of sulfonamide in feed, fish, eggs, honey, meat (beef, chicken and pork), milk, milk powder, serum, shrimp and urine.

The unique features of the kit are:

Rapid extraction method for various samples with high recovery (80-105%).
High sensitivity (0.5 ppb) and low detection limit (0.25 ppb for shrimp).
High reproducibility.
A quick ELISA assay (less than 2 hours regardless of number of samples).

Procedure Overview

The method is based on a competitive colorimetric ELISA assay. The plate wells are coated with capture antibodies directed against anti- sulfonamide antibodies. Sulfonamide standards or sample solution, sulfonamide–HRP conjugate and anti-sulfonamide antibodies are added. Free sulfonamide and sulfonamide-HRP Conjugate compete for the sulfonamide antibody binding sites. At the same time, the anti- sulfonamide antibodies are also bound by the immobilized capture antibodies. Any unbound sulfonamide-HRP Conjugate is then removed in a washing step. The TMB Substrate is added to the wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.

Kit Contents, Storage and Shelf Life

REAGEN^™Sulfonamide ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored.

Kit Contents	Amount	Storage
Sulfonamide Plate	1x96-well Plate(8wellsx12 strips)	2-8°C
SMX Standards: Negative control (white cap tube) 0.5 ng/mL (yellow cap tube) 1.5 ng/mL (orange cap tube) 5.0 ng/mL (pink cap tube) 15 ng/mL (purple cap tube) 50 ng/mL (blue cap tube) 1000 ng/mL(spiking, optional, red cap tube)	1.0 mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL	2-8°C 2-8°C
Sulfonamide Antibody (powder)	2 tubes	2-8°C
Antibody Dilution Buffer	8 mL
Sulfonamide-HRP Conjugate	6 mL
20X Wash Solution **	30 mL
Stop Buffer **	14 mL
TMB Substrate **	10 mL
Concentrate of Tissue Extraction Buffer Tissue Clean Up Mix Honey Extraction Buffer (Optional) 10X Sample Extraction Buffer E	60 g 12 g 3 x 28 mL 25 mL

* If you are not planning to use the kit for over 1 month, store Sulfonamide Antibody, Antibody Dilution Buffer and Sulfonamide -HRP Conjugate at -20°C or in a freezer.

Sensitivity

Sample Type	Detection Limit (ng/g or ppb)
Feed	2.5
Egg	0.25
Fish/Shrimp/Meat/Liver/Kidney	1.0
Honey	1.0
Milk	2.5
Milk Powder	2.0
Serum	2.5
Shrimp	0.25 (Rapid Extraction Method)
Urine	2.5
Water	0.25

Specificity

Analytes	Cross-Reactivity (%)
Sulfamethoxazole (SMX)	100
Sulfapyridine	100
Sulfamethoxypyridazine	100
Sulfaquinoxaline	93
Sulfamethoxydiazine	86
Sulfadiazine	84
Sulfadimethoxine	75
Sulfamethizole	62
Sulfachloropyridazine	58
Sulfamoxole	2
Sulfisoxazole	0.5
Sulfathiazole	<0.1
Sulfamerazine	<0.1
Sulfaquanidine	<0.1
Sulfadoxine	<0.1

Packaging & Shipping

- Packingcolor box
- Delivery Lead Time7 days
- Delivery PortMOORESTOWN
- Shipping Career

Product Image

Sulfonamide Related Products

Company Profile

About Reagen

Membership Free member
Registration Date2013.05.21
Country/RegionUnited States
CityMoorestown
Contact Sia

REAGEN LLC is an indrustry-leading biomedical enterprise focused on designing and producing a broad range of ELISA kits, including clinical diagnostic kits, food and feed safety kits, and veterinary disease kits. we also manufacture microplate readers, plate washers, and related instruments and equipment.

Selling Categories

- Chemicals >> Chemical Reagents

Keywords

elisa kit , elisa test kit

Registration Date	2013/05/21(Year/Month/Date)
Buyer / Seller in EC21	Seller
Business Type	Manufacturer
Year established	2008
Employees total	101 - 500
Annual revenue	USD 100,000 - 500,000

Contact Information Order Credit Report

Company	Reagen
Address	102 Commerce Dr. Unit 8, Moorestown, http://www.reagen.usMoorestownColorado08057United States
Phone	1 - 857 - 7270251
Fax	1 - 857 - 7270251
Homepage	http://WWW.reagen.us
Contact	Sia / mananger

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Sulfonamide ELISA Test Kit

Product Attributes

Description by Manufacturer

Product Description

Procedure Overview

Kit Contents, Storage and Shelf Life

Sensitivity

Specificity

Packaging & Shipping

Product Image

Sulfonamide Related Products

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Company Profile

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