Enrofloxacin ELISA Test Kit

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Price

US$ 300

Min Order Quantity2 Box
Delivery Lead Time7
Payment Terms 1
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Supplier Info.

Company Name Reagen
Membership Free member
Registration Date2013.05.21
Country/RegionUnited States
CityMoorestown
Contact Sia

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Product Attributes

- Brand Name REAGEN
- Model Number RND99041
- Place of Origin United States
- Supply Type In-Stock Items
- Condition New
- Purity 95%
- Related Keywords Enrofloxacin, ELISA Test Kit

Description by Manufacturer

Product Description

REAGEN™Enrofloxacin is a fluoroquinolone that inhibits the activity of bacterial DNA gyrase. It was the first fluoroquinolone antimicrobial to be used in veterinary infections by E. coli, Salmonella, Pasteurella, Mycoplasma and Hemophilus species. The MRLs for the sum of enrofloxacin and its active metabolite ciprofloxacin have been stated at 100 μg/kg (ppb) for muscle tissue and fat, and 100 μg/kg (ppb) for milk. REAGEN™ Enrofloxacin ELISA Test Kit enables government agencies, food manufacturers and quality assurance organizations to detect Enrofloxacin and its main metabolite ciprofloxacin to as low as 0.5 ppb level in various sample types and to satisfy customer concerns about food safety. REAGEN™Enrofloxacin ELISA Test Kit provides a unique extraction method, which is accurate, rapid, cost effective and easy to use. The features of the kit are:

l The extraction of enrofloxacin from feed, honey, meat, milk, tissue, embryonic eggs, serum and urine can be finished in 10-30 minutes with a high recovery rate of 75 - 95%.

l A quick ELISA assay (less than 2 hours regardless of number of samples).

l High reproducibility.

Procedure Overview

The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.

Kit Contents, Storage and Shelf Life

REAGEN™ Enrofloxacin ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C. The shelf life is 12 months when the kit is properly stored.

Kit Contents	Amount	Storage
Enrofloxacin-coated Plate	1 x 96-well plate (8 wells x 12 strips)	2-8°C
Enrofloxacin Standards: Negative control (white cap tube) 0.1 ng/mL (yellow cap tube) 0.25 ng/mL (orange cap tube) 0.5 ng/mL (pink cap tube) 1.0 ng/mL (purple cap tube) 5.0 ng/mL (blue cap tube) 10 ng/mL (spiking, optional, red cap tube)	0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL	2-8°C
Enrofloxacin Antibody #1	12 mL	2-8°C
100X HRP-Conjugated Antibody #2	250 µL
Antibody #2 Diluent **	20 mL
10X Sample Extraction Buffer **	25 mL
20X Wash Solution **	28 mL
Stop Buffer **	14 mL
TMB Substrate **	12 mL
Enro/Cipro Extraction Buffer (Optional)	10 mL
Sample Balance Buffer (Optional)	1.6 mL

*If you are not planning to use the kit for over 3 months, store Enrofloxacin Antibody #1 and 100X HRP-Conjugated Antibody #2 at -20°C preferably or in a freezer.

Sensitivity (Detection Limit)

Sample Type	Detection Limit (ng/g or ppb)
Egg or Feed	1.0
Honey	2.0
Meat/Liver/Kidney/Fish/Shrimp/Embryonic Eggs	1.0
Milk	0.5
Serum	0.5
Urine or Water	0.5

Specificity (Cross-Reactivity)

Enrofloxacin	Cross-Reactivity (%)
Enrofloxacin	100
Ciprofloxacin	100
Danofloxacin	52
Norfloxacin	10
Enoxacin	9
Pipemidic acid	9
Ofloxacin	8
Benofloxacin	7
Flumequin	6
Oxolin acid	5
Nalidixic acid	3

Price

Price US$ 300 / Box

Packaging & Shipping

- Packingcolor box
- Delivery Lead Time7
- Minimum Order 2 Box
- Shipping Career

Product Image

Enrofloxacin Related Products

Company Profile

About Reagen

Membership Free member
Registration Date2013.05.21
Country/RegionUnited States
CityMoorestown
Contact Sia

REAGEN LLC is an indrustry-leading biomedical enterprise focused on designing and producing a broad range of ELISA kits, including clinical diagnostic kits, food and feed safety kits, and veterinary disease kits. we also manufacture microplate readers, plate washers, and related instruments and equipment.

Selling Categories

- Chemicals >> Chemical Reagents

Keywords

elisa kit , elisa test kit

Registration Date	2013/05/21(Year/Month/Date)
Buyer / Seller in EC21	Seller
Business Type	Manufacturer
Year established	2008
Employees total	101 - 500
Annual revenue	USD 100,000 - 500,000

Contact Information Order Credit Report

Company	Reagen
Address	102 Commerce Dr. Unit 8, Moorestown, http://www.reagen.usMoorestownColorado08057United States
Phone	1 - 857 - 7270251
Fax	1 - 857 - 7270251
Homepage	http://WWW.reagen.us
Contact	Sia / mananger

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Enrofloxacin ELISA Test Kit

Product Attributes

Description by Manufacturer

Product Description

Procedure Overview

Kit Contents, Storage and Shelf Life

Price

Packaging & Shipping

Product Image

Enrofloxacin Related Products

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Company Profile

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