Product description
Optimized for extracting RNA from various samples including cultured cells and animal tissue. As lysis time is short, rapid extraction of high-concentration and high-yield RNA from cells and tissues is possible.. Extraction of high-quality, high-purity RNA is possible.
Feature
1) User-friendly silica membrane spin column method
2) Simple lysis protocol, shredder column not used which improves RNA yield
3) No organic solvent precipitation step
4) Excellent quality, extensive validation performed against various samples
5) QC performed 2 times to minimize lot-to-lot variation
6) Instant response to customer inquiries or complaints
Specification
Product name : PURETM Cell&Tissue RNA Extraction Kit
Package : 100 tests/kit
Storage condition : Can be stored at room temperature up to 1 year.
Sample types that can be used : Cultured cells, animal tissue
MOQ : No MOQ required
Certificates of manufacturing facility : ISO9001, ISO14001, ISO13485
Lysis procedure according to sample type
Thorough quality control
Q&A
1. What are the features of the PURE™ series that make it different from other extraction products?
A : Other products use pretreatment(shredder) columns and require two types of spin columns, resulting in a long or complicated extraction process. In addition, some products use organic solvents such as phenol and chloroform. The PURE™ series developed by Infusion Tech does not use pretreatment columns and enables high-purity, high-concentration nucleic acid purification with a single spin column. The precipitation process using organic solvents is also not required, making it a quick and easy three-step process (lysis, wash, elute).
2. What kind of samples can extraction be performed?
A : This kit is optimized for extracting from cultured cells (≤1X107 cells) and tissues(≤30mg).
3. Do I need to grind tissue samples with liquid nitrogen?
A: If the tissue size is large, it is difficult for the buffer to penetrate and lysis efficiency will be significantly reduced. Also, if liquid nitrogen is not used, degradation of RNA is likely to occur. So it is recommended to use liquid nitrogen, mortar and pestle to grind tissue and use the appropriate volume for extraction.
4. Is it possible to perform follow-up experiments (NGS, Sequencing, PCR) with the PURE™ Cell&Tissue RNA Extraction Kit?
A : PURE™ Cell&Tissue RNA Extraction Kit has the advantage of extracting high purity and high concentration of nucleic acids, so it is possible to perform subsequent experiments. Extracted RNA can be used in various experiments including NGS, sequencing, PCR, electroporation, and cloning.
5. Are the components of the PURE™ Cell&Tissue RNA Extraction Kit sterile?
A: The components of the PURE™ Cell&Tissue RNA Extraction Kit are sterile.
6. Which buffers in the PURE™ Cell&Tissue RNA Extraction Kit do I add ethanol to?
A: RB buffer and Wash buffer should be used after adding ethanol. If you want to check for other components, please refer to the MSDS of the product.
7. How is product quality control (QC) performed for the PURE™ Cell&Tissue RNA Extraction Kit?
A: The QC of all products is performed by randomly selecting products of the same LOT after production and performing extraction using our standard sample. The test methods are quantification, electrophoresis, and PCR. Products are released if they meet our criteria.
8. The filter was clogged while using it, why?
A: The amount of sample used to perform extraction is presumed to be high. We recommend re-experimenting within the sample volume range specified in the manual. If the amount of sample is small, it is likely that a low concentration will be measured when quantitating, so please reduce the amount of elution buffer.
9. There are white powder on the wall of the spin column. Is there something wrong with the product?
A : The powder on the wall of the spin column is filter powder caused by static electricity during the manufacturing process. When the solution pass through the filter, the powder will be caught by the filter, and will not influence purity of the eluted RNA.
Introduction of manufacturer
Infusion Tech was founded on 2020. We have focused on developing nucleic acid preparation and molecular diagnosis PCR kits.
We have over 20 patents on novel nucleic acid extraction methods which we have integrated into our lysis buffers of some of our products.
We also have universal extraction kits using silica membrane technology.We have plenty experience performing extraction against various types of research and IVD use samples. Through our combined experience we were able to release high-quality research purpose kits.
Our biggest confidence is in our products quality. We perform extensive quality control, we make sure lot-to-lot variation is minimized.
Many users in Korea have recognized the stable quality of our kits and are changing from market leader kits to ours.
We exhibited our products at many prestigious international medical exhibitions including AACC, MEDICA and MEDLAB.
We welcome you to experience the high quality of our kits.
Manufacturing facility certificates
Manufacturer facilities
International fair exhibition
