Product description
Optimized for extracting RNA from low volumes of
tissue using specially formulated polymers. As lysis
time is short, rapid extraction of high-concentration
and high-purity RNA is possible.
Feature
1) Efficient extraction using specially formulated polymers
2) User-friendly spin column method
3) Simple lysis protocol, shredder column not used which improves RNA yield
4) No organic solvent precipitation step
5) Excellent quality, extensive validation performed against tissue samples
6) QC performed 2 times to minimize lot-to-lot variation
7) Instant response to customer inquiries or complaints
Specification
Product name : PURETM Strong Tissue RNA Extraction Kit
Package : 100 tests/kit
Storage condition : Can be stored at room temperature up to 1 year
Sample types that can be used : animal tissue
MOQ : No MOQ required
Certificates of manufacturing facility : ISO9001, ISO14001, ISO13485
Flow chart
※ When polymers are mixed with samples and vortexing is performed, the polymers physically interact and disrupt plant cell walls of seed and leaves very efficiently.
QC data
Thorough quality control
Q&A
1. Q : What are the features of the PURE™ series that make it different from other extraction products?
A : Other products use pretreatment(shredder) columns and require two types of spin columns, resulting in a long or complicated extraction process. In addition, some products use organic solvents such as phenol and chloroform. The PURE™ series developed by Infusion Tech does not use pretreatment columns and enables high-purity, high-concentration nucleic acid purification with a single spin column. The precipitation process using organic solvents is also not required, making it a quick and easy three-step process (lysis, wash, elute).
2. Q : What is the difference between your PURE™ Cell&Tissue RNA Extraction Kit and the PURE™ Strong Tissue RNA Extraction Kit?
A : The PURE™ Strong Tissue gDNA Extraction Kit has a specially formulated polymer added to the standard product, enhancing extraction efficiency from tissue that are difficult to break down. The lysis efficiency is greatly increased because the polymer can attach to the cell due to the charge difference and disrupts cell walls.
3. Q : How do I use STL buffer?A : Since STL buffer is a specially formulated polymer and has the properties of an insoluble powder, it is natural for precipitates to form. We recommend using the buffer immediately after vortexing, before precipitation occurs.
Q : Is it possible to perform follow-up experiments (NGS, Sequencing, PCR) with the PURE™ Strong Tissue RNA Extraction Kit?A : PURE™ Strong Tissue RNA Extraction Kit has the advantage of extracting high purity and high concentration of nucleic acids, so it is possible to perform subsequent experiments. Extracted RNA can be used in various experiments including NGS, sequencing, PCR, electroporation, and cloning.
4. Q : Are the components of the PURE™ Strong Tissue RNA Extraction Kit sterile?
A : The components of the PURE™ Strong Tissue RNA Extraction Kit are sterile.
5. Q : Which buffers in the PURE™ Strong Tissue RNA Extraction Kit do I add ethanol to?
A : STB buffer and STW buffer should be used after adding ethanol. If you want to check for other components, please refer to the MSDS of the product.
6. Q : How is product quality control (QC) performed for the PURE™ Strong Tissue RNA Extraction Kit?
A : The QC of all products is performed by randomly selecting products of the same LOT after production and performing extraction using our standard sample. The test methods are quantification, electrophoresis, and PCR. Products are released if they meet our criteria.
7. Q : The filter was clogged while using it, why?
A : The amount of sample used to perform extraction is presumed to be high. We recommend re-experimenting within the sample volume range specified in the manual. If the amount of sample is small, it is likely that a low concentration will be measured when quantitating, so please reduce the amount of elution buffer.
8. Q : There are white powder on the wall of the spin column. Is there something wrong with the product?
A : The powder on the wall of the spin column is filter powder caused by static electricity during the manufacturing process. When the solution pass through the filter, the powder will be caught by the filter, and will not influence purity of the eluted RNA.
Introduction of manufacturer
Infusion Tech was founded on 2020. We have focused on developing nucleic acid preparation and molecular diagnosis PCR kits.
We have over 20 patents on novel nucleic acid extraction methods which we have integrated into our lysis buffers of some of our products.
We also have universal extraction kits using silica membrane technology.We have plenty experience performing extraction against various types of research and IVD use samples. Through our combined experience we were able to release high-quality research purpose kits.
Our biggest confidence is in our products quality. We perform extensive quality control, we make sure lot-to-lot variation is minimized.
Many users in Korea have recognized the stable quality of our kits and are changing from market leader kits to ours.
We exhibited our products at many prestigious international medical exhibitions including AACC, MEDICA and MEDLAB.
We welcome you to experience the high quality of our kits.
Manufacturing facility certificates
Manufacturer facilities
International fair exhibition
